OXFORD City conceded late at Chelmsford City to suffer their second successive 1-0 defeat on the road.
Ade Oluwo broke the visitors' hearts in the final minute of normal time to condemn Ross Jenkins’ side to their third straight loss.
It came six days after City went down 1-0 at Chippenham Town, conceding from a penalty deep in stoppage-time.
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New signing Josh Parker started the game after his arrival was announced before kick-off, but the first big chances fell to Chelmsford.
Harry Lodovica saw an early effort cleared off the line and then missed the target when put through one-on-one with Ben Dudzinski.
Parker looked lively and tried his luck several times before the break, while Cambridge United loanee Kai Yearn spurned a good chance in the 69th minute.
A stalemate was on the cards, only for Oluwo to scramble home a late winner.
Meanwhile, Banbury United travel to Peterborough Sports tonight in a clash between the two teams that gained promotion from Pitching In Southern League Premier Central last season.
Both clubs have made a good start to the National League North campaign, sitting second and tenth respectively.
The rearranged Isuzu FA Trophy first qualifying round fixtures also take place tonight.
Didcot Town visit Hamworthy United, Kidlington entertain Tavistock and Thame United welcome Westbury United.
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