Centre back Andy Smith and midfielder Simon Abercrombie will be missing for Oxford City as they visit Dr Martens League Premier outfit Folkestone Invicta in the FA Umbro Trophy first round tonight.
Smith has an Achilles injury, while Abercrombie is to have a precautionary X-ray on his troublesome ankle injury.
To add to City's problems, Michael Thorp is doubtful with a damaged shoulder.
Witney Town will be looking to transfer their impressive away form to Marriotts Stadium when they take on Ryman League Premier club Harrow Borough in the FA Trophy first round.
Teenage defender Ben Johnson is set to make his Thame United debut against Premier side Basingstoke Town in the Ryman League Cup second round at Windmill Stadium.
Johnson comes into the side with skipper Martin Brown (Achilles), Dave Tregurtha (calf) and Andy Williams (thigh) all sidelined.
Striker Wayne Cort returns after failing to arrive on time for Saturday's 3-2 win over Bishop's Stortford after being shaken up in a car crash on the way to Thame.
Jon Corbett and Garry Harrison both face fitness tests on ankle injuries before Banbury United entertain Ilkeston Town in the Dr Martens League Cup first round.
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