Swapping suit trousers for jogging bottoms and smart skirts for tutus helped raise £9,200 for the Oxford Children's Hospital Campaign.
The cash was collected during Wallace and Gromit's Wrong Trousers Day last year, and will help pay for one of the classrooms at the new paediatric unit being built at the John Radcliffe Hospital, in Headington.
The rooms will be used by sick children spending long periods in hospital.
Oxford Children's Hospital Campaign manager Emma Franklin said: "I'm delighted to accept this grant on behalf of the campaign and would like to thank everyone who took part in Wrong Trousers Day last year.
"The money raised will ensure children are not disadvantaged by falling behind in their education.
"Preparatory work on the new Oxford Children's Hospital has already begun, and it is hoped the new hospital will be ready for occupancy in 2006."
Oxford Children's Hospital was one of 23 hospitals and hospices for young people to benefit from last year's Wrong Trousers Day.
Businesses and schools are being encouraged to take part in a similar event on Friday, July 2. Participants will be able to dress normally from the waist up, but for £1 wear the wrong trousers.
Nicola Masters, Director of Wallace & Gromit's Children's Foundation, said: "We're delighted to be able to support Oxford Children's Hospital and their campaign to raise money for the hugely important education facilities at the new hospital which will centralise children's care in Oxfordshire.
"The response to the Wrong Trousers Day campaign last year was fantastic. We're looking forward to working with Oxford again."
Last week, the Oxford Mail revealed that more than £15m had been pledged for the £20m Oxford Children's Hospital, and encouraged readers to get involved to raise the final £2.5m.
To take part in Wrong Trousers Day, log on to www.wrongtrousersday.org
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