Almost 100 people across the Thames Valley were given blood by the air ambulance last year.
96 critically ill or injured patients in Berkshire, Buckinghamshire and Oxfordshire relied on Thames Valley Air Ambulance for life-saving blood donations.
Since becoming an independent provider in 2018, it has given blood to 402 patients.
The information released marks World Blood Donor Day on June 14.
Adam Panter, chief operating officer at Thames Valley Air Ambulance, said: "The ability to give blood to patients who are bleeding heavily, for example after a road traffic collision, is just one of the hospital-level interventions our expert crew can perform at the roadside, or wherever it is needed.
"As a charity, every mission is funded solely by the generosity of the public."
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